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gitlab.mbb.cnrs.fr / edna / exploitation

edna/exploitation/primer_design

How to design a primer ?

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edna/exploitation/primers_specificity_mismatchplot

Visualization of the specificity of designed primers with mismatch plot

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edna/exploitation/edna_intra_pipeline_comparison

This project aims to compare twelve bioinformatics pipelines based on five existing metabarcoding programs to make recommendations for data management in intraspecific variability studies using environmental DNA.

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edna/exploitation/primer_specificity_ecopcr

This project permits to check the specificity of already designed primers.

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edna/exploitation/dada2_and_obitools

This project has for goal to filtrate efficiently eDNA sequences after PCR amplification and NGS, in order to eliminate the false sequences generated by these technologies.

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edna/exploitation/beginning_obitools

The first step to begin with obitools and Bash

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edna/exploitation/swarm_and_obitools

This project is based on the idea that gathering similar sequences allows to faithfully study them by eliminating sequences generated from PCR or NGS errors.

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edna/exploitation/only_obitools

This project presents a pipeline based on OBITools commands in order to eliminate sequences interpreted as coming from PCR errors and to assign each remaining sequence to a taxon thanks to a reference database.

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